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Anti-Human IDH3B Polyclonal Antibody (HT247014)

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Anti-Human IDH3B Polyclonal Antibody
Anti-Human IDH3B Polyclonal Antibody
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Overview
Catalog No.HT247014
Description
Anti-Human IDH3B Polyclonal Antibody (HT247014) is a rabbit polyclonal antibody detecting IDH3B in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human IDH3B (Ser48-Ser385).
Target Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, Isocitric dehydrogenase subunit beta, NAD(+)-specific ICDH subunit beta, IDH3B
Purification Purified by antigen affinity column.
Accession O43837
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial (IDH3B) is a ~42 kDa protein. Plays a structural role to facilitate the assembly and ensure the full activity of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.

Note For research use only.
Images
  • Anti-Human IDH3B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-IDH3B polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Mouse heart lysate
    Lane 3: Rat heart lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human IDH3B Polyclonal Antibody

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