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Anti-Human IFT140 Polyclonal Antibody (HP637014)

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Overview
Catalog No.HP637014
Description
Anti-Human IFT140 Polyclonal Antibody (HP637014) is a rabbit polyclonal antibody detecting IFT140 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human IFT140 (Met1-Ser410).
Target Intraflagellar transport protein 140 homolog, WDTC2, KIAA0590, IFT140, WD and tetratricopeptide repeats protein 2
Purification Purified by antigen affinity column.
Accession Q96RY7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Intraflagellar transport protein 140 homolog (IFT140) is a ~165 kDa protein. Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). Plays a pivotal role in proper development and function of ciliated cells through its role in ciliogenesis and/or cilium maintenance. Required for the development and maintenance of the outer segments of rod and cone photoreceptor cells. Plays a role in maintenance and the delivery of opsin to the outer segment of photoreceptor cells.

1. Mukhopadhyay, S. et al. (2010) Genes & development 24, 2180-93. PMID: 20889716
2. Perrault, I. et al. (2012) American journal of human genetics 90, 864-70. PMID: 22503633
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human IFT140 Polyclonal Antibody

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