Anti-Human IFT20 Polyclonal Antibody (HP142014)

Name: Anti-Human IFT20 Polyclonal Antibody
Brand: abinScience
SKU: HP142014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP142014

Description

Anti-Human IFT20 Polyclonal Antibody (HP142014) is a rabbit polyclonal antibody detecting IFT20 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Danio rerio, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IFT20 (Met1-Lys132).

Target

hIFT20, IFT20, Intraflagellar transport protein 20 homolog

Purification

Purified by antigen affinity column.

Accession

Q8IY31

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Intraflagellar transport protein 20 homolog (IFT20) is a ~15 kDa protein. Part of intraflagellar transport (IFT) particles involved in ciliary process assembly. May play a role in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium. Regulates the platelet-derived growth factor receptor-alpha (PDGFRA) signaling pathway. Required for protein stability of E3 ubiquitin ligases CBL and CBLB that mediate ubiquitination and internalization of PDGFRA for proper feedback inhibition of PDGFRA signaling. Essential for male fertility.

1. Pugacheva, EN. et al. (2007) Cell 129, 1351-63. PMID: 17604723

2. Follit, JA. et al. (2006) Molecular biology of the cell 17, 3781-92. PMID: 16775004

3. Schmid, FM. et al. (2018) The Journal of cell biology 217, 151-161. PMID: 29237719

Note

For research use only.

Images

References

Datasheet

Anti-Human IFT20 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human IFT20 Polyclonal Antibody (HP142014)

Overview

Images

References

Datasheet

SDS

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