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Anti-Human IFT81 Polyclonal Antibody (HP940014)

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Overview
Catalog No.HP940014
Description
Anti-Human IFT81 Polyclonal Antibody (HP940014) is a rabbit polyclonal antibody detecting IFT81 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human IFT81 (Met1-Lys124).
Target CDV1, IFT81, CDV-1, Carnitine deficiency-associated protein expressed in ventricle 1, Intraflagellar transport protein 81 homolog
Purification Purified by antigen affinity column.
Accession Q8WYA0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Intraflagellar transport protein 81 homolog (IFT81) is a ~79 kDa protein. Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its CH (calponin-homology)-like region. Required for ciliogenesis. Required for proper regulation of SHH signaling. Plays an important role during spermatogenesis by modulating the assembly and elongation of the sperm flagella.

1. Bhogaraju, S. et al. (2013) Science (New York, N.Y.) 341, 1009-12. PMID: 23990561
2. Duran, I. et al. (2016) Scientific reports 6, 34232. PMID: 27666822
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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