Anti-Human IKBKB Polyclonal Antibody (HT056014)

Name: Anti-Human IKBKB Polyclonal Antibody
Brand: abinScience
SKU: HT056014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HT056014

Description

Anti-Human IKBKB Polyclonal Antibody (HT056014) is a rabbit polyclonal antibody detecting IKBKB in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human IKBKB (Glu16-Gly345).

Target

IKKB, IKK-beta, Serine/threonine protein kinase IKBKB, I-kappa-B-kinase beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B kinase 2, IKBKB, IKK-B, NFKBIKB, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, IkBKB

Purification

Purified by antigen affinity column.

Accession

O14920

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Inhibitor of nuclear factor kappa-B kinase subunit beta (IKBKB) is a ~86 kDa protein. Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation. Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis.

1. Cui, J. et al. (2010) Cell 141, 483-96. PMID: 20434986

2. Tsuchiya, Y. et al. (2010) Molecular cell 39, 570-82. PMID: 20797629

3. Clark, K. et al. (2011) The Biochemical journal 434, 93-104. PMID: 21138416

4. Cardinez, C. et al. (2018) The Journal of experimental medicine 215, 2715-2724. PMID: 30337470

5. Mercurio, F. et al. (1997) Science (New York, N.Y.) 278, 860-6. PMID: 9346484

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human IKBKB polyclonal antibody at 1ug/mL on recombinant Human IKBKB (Catalog No. HT056012).
Western blot

Western blot analysis was performed using anti-IKBKB polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

References

Datasheet

Anti-Human IKBKB Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human IKBKB Polyclonal Antibody (HT056014)

Overview

Images

References

Datasheet

SDS

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