Anti-Human IL-10 Recombinant Antibody (hum12G8) (HB997013)

Name: Anti-Human IL-10 Recombinant Antibody (hum12G8)
Brand: abinScience
SKU: HB997013
Price: 110 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB997013

Species reactivity

Human

Applications

Blocking, ELISA

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG1

Clone ID

hum12G8

Target

CSIF, Cytokine synthesis inhibitory factor, IL-10, IL10, Interleukin-10

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P22301

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Interleukin-10 (IL10) is a ~20 kDa protein. Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF. Also interferes with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation.

1. Yoon, SI. et al. (2006) The Journal of biological chemistry 281, 35088-96. PMID: 16982608

2. El Kasmi, KC. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 7215-9. PMID: 18025162

3. Hashimoto, SI. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 167, 3619-25. PMID: 11564774

4. de Waal Malefyt, R. et al. (1991) The Journal of experimental medicine 174, 1209-20. PMID: 1940799

5. Willems, F. et al. (1994) European journal of immunology 24, 1007-9. PMID: 7512027

6. Frei, K. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 2720-8. PMID: 8144879

Note

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

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