Anti-Human IL25/IL17E Recombinant Antibody (2c3) (HV984013)

Name: Anti-Human IL25/IL17E Recombinant Antibody (2c3)
Brand: abinScience
SKU: HV984013
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV984013

Species reactivity

Human

Applications

ELISA, FCM

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

2c3

Clonality

Monoclonal

Target

Interleukin-25, IL-25, IL17E, IL25, IL-17E, Interleukin-17E

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9H293

RRID

Anti-Human IL25/IL17E Antibody (2c3) (abinScience Cat# HV984013, RRID:AB_3725663)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Interleukin-25 (IL25/IL17E) is a ~20 kDa protein. Cytokine produced by various cells such as eosinophils, T-helper type 2 (Th2) cells or epithelial cells that plays a role in internal safety of adaptive immune responses by regulating cytokine production. Promotes and augments T-helper type 2 responses locally or systemically. Exerts its activity via its receptor composed of IL17RA and IL17RB for signal transduction. In turn, stimulates the JAK2-STAT5A pathway and promotes the secretion of type-2 associated cytokines including IL4, IL9 and IL13. Also induces the release of IL8, and IL6 from eosinophils through the combined activation of MAPK and NF-kappa-B pathways.

1. Wong, CK. et al. (2005) American journal of respiratory cell and molecular biology 33, 186-94. PMID: 15860795

2. Wu, L. et al. (2015) Journal of immunology (Baltimore, Md. : 1950) 194, 4528-34. PMID: 25821217

3. Fort, MM. et al. (2001) Immunity 15, 985-95. PMID: 11754819

Note

For research use only.

Images

Bioactivity

Detects Human IL25/IL17E in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human IL25 antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL25 antibody monoclonal antibody (Catalog # HV984013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human IL25/IL17E Antibody (2c3).