Anti-Human IL3 Recombinant Antibody (SAA1520) (HY262013)

Name: Anti-Human IL3 Recombinant Antibody (SAA1520)
Brand: abinScience
SKU: HY262013
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY262013

Species reactivity

Human

Applications

ELISA, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1520

Clonality

Monoclonal

Target

IL3, Hematopoietic growth factor, Multipotential colony-stimulating factor, IL-3, Mast cell growth factor, Interleukin-3, P-cell-stimulating factor, MCGF

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08700

RRID

Anti-Human IL3 Antibody (SAA1520) (abinScience Cat# HY262013, RRID:AB_3727281)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Interleukin-3 (IL3) is a ~17 kDa protein. Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB.

1. Guba, SC. et al. (1989) The Journal of clinical investigation 84, 1701-6. PMID: 2556442

2. Okayama, Y. et al. (1993) Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 23, 901-10. PMID: 10779277

3. Hong, L. et al. (2020) Cytokine 136, 155268. PMID: 32889153

4. Luo, XJ. et al. (2012) PloS one 7, e50375. PMID: 23226269

5. Khapli, SM. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 171, 142-51. PMID: 12816992

6. Broughton, SE. et al. (2018) Nature communications 9, 386. PMID: 29374162

7. López, C. et al. (2017) Journal of cellular biochemistry 118, 1330-1340. PMID: 27862234

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human IL3 Antibody (SAA1520)
Bioactivity

Detects recombinant human IL3 (Catalog No: HY262012) in indirect ELISA.
Bioactivity

Detects recombinant human IL3 (Catalog No: HY262011) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-IL3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL3 (Catalog No: HY262011)