Anti-Human IPO4 Polyclonal Antibody (HP026014)

Name: Anti-Human IPO4 Polyclonal Antibody
Brand: abinScience
SKU: HP026014
Price: 96 USD
Availability: InStock

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References
Datasheet
SDS

Overview

Catalog No.

HP026014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human IPO4 (Asn667-Ser1081).

Target

RanBP4, Imp4b, Importin-4b, Ran-binding protein 4, RANBP4, IPO4, Importin-4, Imp4, IMP4B

Purification

Purified by antigen affinity column.

Accession

Q8TEX9

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Importin-4 (IPO4) is a ~118 kDa protein. Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

1. Jäkel, S. et al. (2002) The EMBO journal 21, 377-86. PMID: 11823430

2. Miyauchi, Y. et al. (2005) The Journal of biological chemistry 280, 40901-8. PMID: 16207705

3. Pradeepa, MM. et al. (2008) Molecular and cellular biology 28, 4331-41. PMID: 17682055

4. Alvarez, F. et al. (2011) The Journal of biological chemistry 286, 17714-21. PMID: 21454524

5. Apta-Smith, MJ. et al. (2018) The EMBO journal 37. PMID: 30177573

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-IPO4 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: Lncap cell lysate
Lane 3: A549 cell lysate

References

Datasheet

Anti-Human IPO4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human IPO4 Polyclonal Antibody (HP026014)

Overview

Images

References

Datasheet

SDS

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