Anti-Human ISCU Polyclonal Antibody (HC184014)

Name: Anti-Human ISCU Polyclonal Antibody
Brand: abinScience
SKU: HC184014
Price: 168 USD
Availability: InStock

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References
Datasheet
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Overview

Catalog No.

HC184014

Description

Anti-Human ISCU Polyclonal Antibody (HC184014) is a rabbit polyclonal antibody detecting ISCU. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ISCU (His36-Lys167).

Target

ISCU, Iron-sulfur cluster assembly enzyme ISCU, NIFUN, NifU-like N-terminal domain-containing protein, NifU-like protein

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9H1K1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Iron-sulfur cluster assembly enzyme ISCU is a ~17 kDa protein. Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (Probable).

1. Freibert, SA. et al. (2021) Nature communications 12, 6902. PMID: 34824239

2. Bridwell-Rabb, J. et al. (2014) Biochemistry 53, 4904-13. PMID: 24971490

3. Cai, K. et al. (2018) Journal of inorganic biochemistry 183, 107-116. PMID: 29576242

4. Fox, NG. et al. (2018) Biochimie 152, 211-218. PMID: 30031876

5. Cai, K. et al. (2013) The Journal of biological chemistry 288, 28755-70. PMID: 23940031

Note

For research use only

Images

References

Datasheet

Anti-Human ISCU Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ISCU Polyclonal Antibody (HC184014)

Overview

Images

References

Datasheet

SDS

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