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Anti-Human ISYNA1 Polyclonal Antibody (HP004014)

Anti-Human ISYNA1 Polyclonal Antibody
Anti-Human ISYNA1 Polyclonal Antibody
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Overview
Catalog No.HP004014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ISYNA1 (Met1-Gly495).
Target hIPS, Myo-inositol 1-phosphate synthase, Inositol-3-phosphate synthase 1, INO1, ISYNA1, Myo-inositol 1-phosphate synthase A1, MIP synthase, IPS 1, hINO1, MI-1-P synthase
Purification Purified by antigen affinity column.
Accession Q9NPH2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Inositol-3-phosphate synthase 1 (ISYNA1) is a ~61 kDa protein. Key enzyme in myo-inositol biosynthesis pathway that catalyzes the conversion of glucose 6-phosphate to 1-myo-inositol 1-phosphate in a NAD-dependent manner. Rate-limiting enzyme in the synthesis of all inositol-containing compounds.

1. Ju, S. et al. (2004) The Journal of biological chemistry 279, 21759-65. PMID: 15024000
2. Deranieh, RM. et al. (2013) The Journal of biological chemistry 288, 26822-33. PMID: 23902760
Note For research use only.
Images
  • Anti-Human ISYNA1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ISYNA1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: K562 cell lysate
    Lane 2: HCT-116 cell lysate
    Lane 3: Mouse testis lysate
    Lane 4: Rat testis lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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