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Anti-Human JMJD7 Polyclonal Antibody (HY049014)

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Overview
Catalog No.HY049014
Description
Anti-Human JMJD7 Polyclonal Antibody (HY049014) is a rabbit polyclonal antibody detecting JMJD7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human JMJD7 (Met1-Asp316).
Target Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7, 1.14.11.63, 3.4.-.-, JmjC domain-containing protein 7, Jumonji domain-containing protein 7, L-lysine (3S)-hydroxylase JMJD7, JMJD7
Purification Purified by antigen affinity column.
Accession P0C870
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Bifunctional peptidase and (3S)-lysyl hydroxylase JMJD7 is a ~35 kDa protein. Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monooxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Additionally, may play a role in protein biosynthesis by modifying the translation machinery.

1. Liu, H. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E7717-E7726. PMID: 28847961
2. Markolovic, S. et al. (2018) Nature chemical biology 14, 688-695. PMID: 29915238
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human JMJD7 Polyclonal Antibody

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