Anti-Human KCNH2/ERG1 Polyclonal Antibody (HC535014)

Name: Anti-Human KCNH2/ERG1 Polyclonal Antibody
Brand: abinScience
SKU: HC535014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC535014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KCNH2/ERG1 (Pro10-Lys135).

Target

H-ERG, ERG, HERG, hERG1, hERG-1, Potassium voltage-gated channel subfamily H member 2, ERG-1, KCNH2, Eag homolog, ERG1, Eag-related protein 1, Ether-a-go-go-related gene potassium channel 1, Voltage-gated potassium channel subunit Kv11.1, Ether-a-go-go-related protein 1

Purification

Purified by antigen affinity column.

Accession

Q12809

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Voltage-gated inwardly rectifying potassium channel KCNH2 (ERG1) is a ~126 kDa protein. Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Characterized by unusual gating kinetics by producing relatively small outward currents during membrane depolarization and large inward currents during subsequent repolarization which reflect a rapid inactivation during depolarization and quick recovery from inactivation but slow deactivation (closing) during repolarization. Forms a stable complex with KCNE1 or KCNE2, and that this heteromultimerization regulates inward rectifier potassium channel activity.

1. Abbott, GW. et al. (1999) Cell 97, 175-87. PMID: 10219239

2. Kagan, A. et al. (2000) The Journal of biological chemistry 275, 11241-8. PMID: 10753933

3. Paulussen, A. et al. (2000) Human mutation 15, 483. PMID: 10790218

4. Cui, J. et al. (2000) Current biology : CB 10, 671-4. PMID: 10837251

5. Yang, P. et al. (2002) Circulation 105, 1943-8. PMID: 11997281

6. Gong, Q. et al. (2002) American journal of physiology. Heart and circulatory physiology 283, H77-84. PMID: 12063277

7. Guasti, L. et al. (2008) Molecular and cellular biology 28, 5043-60. PMID: 18559421

8. Aidery, P. et al. (2012) Biochemical and biophysical research communications 418, 830-5. PMID: 22314138

9. Ng, CA. et al. (2012) PloS one 7, e31640. PMID: 22359612

10. Kang, Y. et al. (2015) The Biochemical journal 472, 71-82. PMID: 26363003

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-KCNH2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate