Anti-Human KDM1A Polyclonal Antibody (HD579014)

Name: Anti-Human KDM1A Polyclonal Antibody
Brand: abinScience
SKU: HD579014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HD579014

Description

Anti-Human KDM1A Polyclonal Antibody (HD579014) is a rabbit polyclonal antibody detecting KDM1A. Suitable for Rat, Mouse, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KDM1A (Arg524-Met852).

Target

AOF2, BHC110, BRAF35-HDAC complex protein BHC110, EC:1.14.11.-, EC:1.14.11.65, EC:1.14.99.66, Flavin-containing amine oxidase domain-containing protein 2, KDM1, KDM1A, KIAA0601, LSD1, Lysine-specific histone demethylase 1A, [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O60341

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Lysine-specific histone demethylase 1A (KDM1A) is a ~92 kDa protein. Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2). May play a role in the repression of neuronal genes.

1. Shi, Y. et al. (2004) Cell 119, 941-53. PMID: 15620353

2. Forneris, F. et al. (2005) FEBS letters 579, 2203-7. PMID: 15811342

3. Lee, MG. et al. (2005) Nature 437, 432-5. PMID: 16079794

4. Metzger, E. et al. (2005) Nature 437, 436-9. PMID: 16079795

5. Shi, YJ. et al. (2005) Molecular cell 19, 857-64. PMID: 16140033

6. Forneris, F. et al. (2005) The Journal of biological chemistry 280, 41360-5. PMID: 16223729

7. Luo, H. et al. (2016) Cell reports 15, 2665-78. PMID: 27292636

10. Lin, Y. et al. (2010) The EMBO journal 29, 1803-16. PMID: 20389281

Note

For research use only

Images

References

Datasheet

Anti-Human KDM1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human KDM1A Polyclonal Antibody (HD579014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us