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Anti-Human KDM4E Polyclonal Antibody (HA063014)

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Overview
Catalog No.HA063014
Description
Anti-Human KDM4E Polyclonal Antibody (HA063014) is a rabbit polyclonal antibody detecting KDM4E in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human KDM4E (Thr14-Arg372).
Target Lysine-specific demethylase 4E, 1.14.11.66, KDM4D-like protein, Lysine-specific demethylase 4D-like, [histone H3]-trimethyl-L-lysine(9) demethylase 4E, KDM4E, KDM4DL
Purification Purified by antigen affinity column.
Accession B2RXH2
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Lysine-specific demethylase 4E (KDM4E) is a ~56 kDa protein. Histone demethylase that demethylates trimethylated 'Lys-9' of histone H3 into monomethylated form, thereby playing a central role in histone code. Also demethylates trimethylated 'Lys-56' of histone H3 (H3K9me3) to generate the monomethylated state (H3K56me1).

1. Hillringhaus, L. et al. (2011) The Journal of biological chemistry 286, 41616-41625. PMID: 21914792
2. Jack, AP. et al. (2013) PloS one 8, e51765. PMID: 23451023
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human KDM4E Polyclonal Antibody

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