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Anti-Human KIF3A Polyclonal Antibody (HP882014)

Anti-Human KIF3A Polyclonal Antibody
Anti-Human KIF3A Polyclonal Antibody
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Overview
Catalog No.HP882014
Description
Anti-Human KIF3A Polyclonal Antibody (HP882014) is a rabbit polyclonal antibody detecting KIF3A in ELISA, IHC, WB. Suitable for Human, Mouse, Macaca fascicularis, and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human KIF3A (Lys16-Ile345).
Target Microtubule plus end-directed kinesin motor 3A, Kinesin-like protein KIF3A, KIF3A, KIF3
Purification Purified by antigen affinity column.
Accession Q9Y496
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Kinesin-like protein KIF3A is a ~80 kDa protein. Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole.

Note For research use only.
Images
  • Anti-Human KIF3A Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-KIF3A polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse brian lysate
    Lane 2: Mouse cerebellum lysate
    Lane 3: Rat brian lysate
    Lane 4: Rat cerebellum lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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