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Anti-Human KLHL22 Polyclonal Antibody (HS782014)

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Overview
Catalog No.HS782014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human KLHL22 (Asp296-Thr592).
Target Kelch-like protein 22, KLHL22
Purification Purified by antigen affinity column.
Accession Q53GT1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Kelch-like protein 22 (KLHL22) is a ~71 kDa protein. Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway.

1. Maerki, S. et al. (2009) The Journal of cell biology 187, 791-800. PMID: 19995937
2. Beck, J. et al. (2013) Nature cell biology 15, 430-9. PMID: 23455478
3. Chen, J. et al. (2018) Nature 557, 585-589. PMID: 29769719
Note For research use only.
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Formula
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Anti-Human KLHL22 Polyclonal Antibody

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