Anti-Human KMT5A Polyclonal Antibody (HP554014)

Name: Anti-Human KMT5A Polyclonal Antibody
Brand: abinScience
SKU: HP554014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HP554014

Description

Anti-Human KMT5A Polyclonal Antibody (HP554014) is a rabbit polyclonal antibody detecting KMT5A in ELISA, IHC, WB. Suitable for Bovine, Human, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KMT5A (Leu263-His393).

Target

Histone-lysine N-methyltransferase KMT5A, PR/SET07, SETD8, SET8, PR-Set7, Lysine N-methyltransferase 5A, PRSET7, N-lysine methyltransferase KMT5A, SET domain-containing protein 8, Lysine-specific methylase 5A, H4-K20-HMTase KMT5A, SET07, KMT5A, PR/SET domain-containing protein 07

Purification

Purified by antigen affinity column.

Accession

Q9NQR1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

N-lysine methyltransferase KMT5A is a ~42 kDa protein. Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis.

1. Nishioka, K. et al. (2002) Molecular cell 9, 1201-13. PMID: 12086618

2. Fang, J. et al. (2002) Current biology : CB 12, 1086-99. PMID: 12121615

3. Yin, Y. et al. (2005) The Journal of biological chemistry 280, 30025-31. PMID: 15964846

4. Shi, X. et al. (2007) Molecular cell 27, 636-46. PMID: 17707234

5. Saredi, G. et al. (2016) Nature 534, 714-718. PMID: 27338793

6. Julien, E. et al. (2004) Molecular cell 14, 713-25. PMID: 15200950

7. Xiao, B. et al. (2005) Genes & development 19, 1444-54. PMID: 15933069

8. Couture, JF. et al. (2005) Genes & development 19, 1455-65. PMID: 15933070

Note

For research use only.

Images

References

Datasheet

Anti-Human KMT5A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human KMT5A Polyclonal Antibody (HP554014)

Overview

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References

Datasheet

SDS

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