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Anti-Human KRT2 Polyclonal Antibody (HW429014)

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Overview
Catalog No.HW429014
Description
Anti-Human KRT2 Polyclonal Antibody (HW429014) is a rabbit polyclonal antibody detecting KRT2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human KRT2 (Asn342-Leu495).
Target Keratin, type II cytoskeletal 2 epidermal, Cytokeratin-2e, CK-2e, Epithelial keratin-2e, Keratin-2 epidermis, Keratin-2e, K2e, Type-II keratin Kb2, KRT2, KRT2A, KRT2E
Purification Purified by antigen affinity column.
Accession P35908
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Keratin, type II cytoskeletal 2 epidermal (KRT2) is a ~65 kDa protein. Probably contributes to terminal cornification. Associated with keratinocyte activation, proliferation and keratinization. Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins. Plays a role in the establishment of the epidermal barrier on plantar skin.

1. Collin, C. et al. (1992) Experimental cell research 202, 132-41. PMID: 1380918
2. Bloor, BK. et al. (2003) The American journal of pathology 162, 963-75. PMID: 12598329
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human KRT2 Polyclonal Antibody

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