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Anti-Human LAGE3 Polyclonal Antibody (HB516014)

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Overview
Catalog No.HB516014
Description
Anti-Human LAGE3 Polyclonal Antibody (HB516014) is a rabbit polyclonal antibody detecting EKC/KEOPS complex subunit LAGE3; L antigen family member 3; Protein ESO-3; Protein ITBA2; LAGE3; DXS9879E; ESO3; ITBA2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human LAGE3 (Met58-Arg143).
Target EKC/KEOPS complex subunit LAGE3; L antigen family member 3; Protein ESO-3; Protein ITBA2; LAGE3; DXS9879E; ESO3; ITBA2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q14657
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

EKC/KEOPS complex subunit LAGE3 is a ~14 kDa protein. Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. LAGE3 functions as a dimerization module for the complex.

1. Costessi, A. et al. (2012) PloS one 7, e42822. PMID: 22912744
2. Wan, LC. et al. (2017) Nucleic acids research 45, 805-817. PMID: 27903914
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human LAGE3 Polyclonal Antibody

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