Anti-Human LAMTOR5/HBXIP Polyclonal Antibody (HT273014)

Name: Anti-Human LAMTOR5/HBXIP Polyclonal Antibody
Brand: abinScience
SKU: HT273014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HT273014

Description

Anti-Human LAMTOR5/HBXIP Polyclonal Antibody (HT273014) is a rabbit polyclonal antibody detecting LAMTOR5. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LAMTOR5/HBXIP (Met1-Ser91).

Target

HBV X-interacting protein, HBX-interacting protein, HBXIP, Hepatitis B virus X-interacting protein, LAMTOR5, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 5, Ragulator complex protein LAMTOR5, XIP

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O43504

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Ragulator complex protein LAMTOR5 (HBXIP) is a ~9 kDa protein. As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. When complexed to BIRC5, interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby selectively suppressing apoptosis initiated via the mitochondrial/cytochrome c pathway.

1. Bar-Peled, L. et al. (2012) Cell 150, 1196-208. PMID: 22980980

2. Yonehara, R. et al. (2017) Nature communications 8, 1625. PMID: 29158492

3. Shen, K. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 9545-9550. PMID: 30181260

4. de Araujo, MEG. et al. (2017) Science (New York, N.Y.) 358, 377-381. PMID: 28935770

5. Su, MY. et al. (2017) Molecular cell 68, 835-846.e3. PMID: 29107538

Note

For research use only

Images

References

Datasheet

Anti-Human LAMTOR5/HBXIP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human LAMTOR5/HBXIP Polyclonal Antibody (HT273014)

Overview

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References

Datasheet

SDS

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