Anti-Human LATS1 Polyclonal Antibody (HA517014)

Name: Anti-Human LATS1 Polyclonal Antibody
Brand: abinScience
SKU: HA517014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HA517014

Description

Anti-Human LATS1 Polyclonal Antibody (HA517014) is a rabbit polyclonal antibody detecting LATS1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LATS1 (Ser635-Tyr1076).

Target

LATS1

Purification

Purified by antigen affinity column.

Accession

O95835

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Serine/threonine-protein kinase LATS1, 2.7.11.1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, LATS1, WARTS

Background

Serine/threonine-protein kinase LATS1 is a ~126 kDa protein. Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity.

1. Nishiyama, Y. et al. (1999) FEBS letters 459, 159-65. PMID: 10518011

2. Hirota, T. et al. (2000) The Journal of cell biology 149, 1073-86. PMID: 10831611

3. Hao, Y. et al. (2008) The Journal of biological chemistry 283, 5496-509. PMID: 18158288

4. Meng, Z. et al. (2015) Nature communications 6, 8357. PMID: 26437443

5. Britschgi, A. et al. (2017) Nature 541, 541-545. PMID: 28068668

10. Nie, L. et al. (2024) Molecular cell 84, 3336-3353.e7. PMID: 39173637

Note

For research use only.

Images

References

Datasheet

Anti-Human LATS1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human LATS1 Polyclonal Antibody (HA517014)

Overview

Images

References

Datasheet

SDS

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