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Anti-Human LCP1 Polyclonal Antibody (HB678024)

Anti-Human LCP1 Polyclonal Antibody
Anti-Human LCP1 Polyclonal Antibody
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Overview
Catalog No.HB678024
Description
Anti-Human LCP1 Polyclonal Antibody (HB678024) is a rabbit polyclonal antibody detecting LCP1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human LCP1 (Ser5-Lys233).
Target LCP1
Purification Purified by antigen affinity column.
Accession P13796
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesPlastin-2, L-plastin, LC64P, Lymphocyte cytosolic protein 1, LCP-1, LCP1, PLS2
Background

Plastin-2 (LCP1) is a ~70 kDa protein. Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.

1. Janji, B. et al. (2006) Journal of cell science 119, 1947-60. PMID: 16636079
2. Wabnitz, GH. et al. (2007) European journal of immunology 37, 649-62. PMID: 17294403
3. Rainger, J. et al. (2017) Human mutation 38, 942-946. PMID: 28493397
Note For research use only.
Images
  • Anti-Human LCP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-LCP1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: K562 cell lysate
    Lane 3: THP-1 cell lysate
    Lane 4: HL-60 cell lysate
    Lane 5: Raji cell lysate
    Lane 6: RAW264.7 cell lysate
    Lane 7: Mouse spleen lysate
    Lane 8: Rat spleen lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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