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Anti-Human LCT Polyclonal Antibody (HY498014)

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Overview
Catalog No.HY498014
Description
Anti-Human LCT Polyclonal Antibody (HY498014) is a rabbit polyclonal antibody detecting LCT in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human LCT (Ser20-Gly336).
Target Lactase/phlorizin hydrolase, Lactase/glycosylceramidase, Lactase, 3.2.1.108, Glycosylceramidase, 3.2.1.62, Phlorizin hydrolase, LCT, LPH
Purification Purified by antigen affinity column.
Accession P09848
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Lactase/phlorizin hydrolase (LCT) is a ~218 kDa protein. Broad specificity glycosidase of the intestinal brush border membrane that hydrolyzes lactose, the main sugar in mammalian milk, to produce D-glucose and D-galactose. The mature protein is composed of two domains that catalyze the hydrolysis of beta-glucopyranosides and beta-galactopyranosides, with a preference for hydrophilic aglycones (in lactose and cellobiose) for one domain and hydrophobic aglycones (in phlorizin and glycosylceramides) for the other.

1. Németh, K. et al. (2003) European journal of nutrition 42, 29-42. PMID: 12594539
2. Kuokkanen, M. et al. (2006) American journal of human genetics 78, 339-44. PMID: 16400612
3. Potter, J. et al. (1985) Biochemical genetics 23, 423-39. PMID: 3929764
4. Zecca, L. et al. (1998) FEBS letters 435, 225-8. PMID: 9762914
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human LCT Polyclonal Antibody

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