Anti-Human LCT Polyclonal Antibody (HY498014)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY498014

Description

Anti-Human LCT Polyclonal Antibody (HY498014) is a rabbit polyclonal antibody detecting LCT in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LCT (Ser20-Gly336).

Target

Lactase/phlorizin hydrolase, Lactase/glycosylceramidase, Lactase, 3.2.1.108, Glycosylceramidase, 3.2.1.62, Phlorizin hydrolase, LCT, LPH

Purification

Purified by antigen affinity column.

Accession

P09848

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Lactase/phlorizin hydrolase (LCT) is a ~218 kDa protein. Broad specificity glycosidase of the intestinal brush border membrane that hydrolyzes lactose, the main sugar in mammalian milk, to produce D-glucose and D-galactose. The mature protein is composed of two domains that catalyze the hydrolysis of beta-glucopyranosides and beta-galactopyranosides, with a preference for hydrophilic aglycones (in lactose and cellobiose) for one domain and hydrophobic aglycones (in phlorizin and glycosylceramides) for the other.

1. Németh, K. et al. (2003) European journal of nutrition 42, 29-42. PMID: 12594539

2. Kuokkanen, M. et al. (2006) American journal of human genetics 78, 339-44. PMID: 16400612

3. Potter, J. et al. (1985) Biochemical genetics 23, 423-39. PMID: 3929764

4. Zecca, L. et al. (1998) FEBS letters 435, 225-8. PMID: 9762914

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human LCT polyclonal antibody at 1ug/mL on recombinant Human LCT (Catalog No. HY498012).

References

Datasheet

Anti-Human LCT Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human LCT Polyclonal Antibody (HY498014)

Overview

Images

References

Datasheet

SDS

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