Anti-Human LIMK2 Polyclonal Antibody (HW771014)

Name: Anti-Human LIMK2 Polyclonal Antibody
Brand: abinScience
SKU: HW771014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HW771014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LIMK2 (Asp330-Pro638).

Target

LIM domain kinase 2, LIMK-2, LIMK2

Purification

Purified by antigen affinity column.

Accession

P53671

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

LIM domain kinase 2 (LIMK2) is a ~72 kDa protein. Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways; phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1.

1. Maekawa, M. et al. (1999) Science (New York, N.Y.) 285, 895-8. PMID: 10436159

2. Sumi, T. et al. (2001) The Journal of biological chemistry 276, 670-6. PMID: 11018042

3. Heng, YW. et al. (2012) Journal of cell science 125, 1579-90. PMID: 22328514

4. Okano, I. et al. (1995) The Journal of biological chemistry 270, 31321-30. PMID: 8537403

5. Kim, J. et al. (2015) Nature communications 6, 6781. PMID: 25849865

Note

For research use only.

Images

References

Datasheet

Anti-Human LIMK2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human LIMK2 Polyclonal Antibody (HW771014)

Overview

Images

References

Datasheet

SDS

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