Anti-Human LMNA Polyclonal Antibody (HA509014)

Name: Anti-Human LMNA Polyclonal Antibody
Brand: abinScience
SKU: HA509014
Price: 96 USD
Availability: InStock

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SDS

Overview

Catalog No.

HA509014

Description

Anti-Human LMNA Polyclonal Antibody (HA509014) is a rabbit polyclonal antibody detecting LMNA in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Sus scrofa, and Gallus gallus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human LMNA (Thr27-Ile229).

Target

LMNA

Purification

Purified by antigen affinity column.

Accession

P02545

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Prelamin-A/C, Lamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1

Background

Prelamin-A/C (LMNA) is a ~74 kDa protein. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. Lamin A and C are present in equal amounts in the lamina of mammals.

1. Bonne, G. et al. (1999) Nature genetics 21, 285-8. PMID: 10080180

2. Fatkin, D. et al. (1999) The New England journal of medicine 341, 1715-24. PMID: 10580070

3. Cao, H. et al. (2000) Human molecular genetics 9, 109-12. PMID: 10587585

4. Muchir, A. et al. (2000) Human molecular genetics 9, 1453-9. PMID: 10814726

5. De Sandre-Giovannoli, A. et al. (2002) American journal of human genetics 70, 726-36. PMID: 11799477

6. Novelli, G. et al. (2002) American journal of human genetics 71, 426-31. PMID: 12075506

7. Chen, L. et al. (2003) Lancet (London, England) 362, 440-5. PMID: 12927431

8. Navarro, CL. et al. (2004) Human molecular genetics 13, 2493-503. PMID: 15317753

9. Quijano-Roy, S. et al. (2008) Annals of neurology 64, 177-86. PMID: 18551513

10. Renou, L. et al. (2008) Journal of medical genetics 45, 666-71. PMID: 18611980

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-LMNA polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: A375 cell lysate

References

Datasheet

Anti-Human LMNA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human LMNA Polyclonal Antibody (HA509014)

Overview

Images

References

Datasheet

SDS

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