Anti-Human LTA4H Polyclonal Antibody (HY412014)

Name: Anti-Human LTA4H Polyclonal Antibody
Brand: abinScience
SKU: HY412014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY412014

Description

Anti-Human LTA4H Polyclonal Antibody (HY412014) is a rabbit polyclonal antibody detecting LTA4H in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human LTA4H (Met1-Leu210).

Target

Leukotriene A-4 hydrolase, LTA-4 hydrolase, 3.3.2.6, Leukotriene A(4) hydrolase, Tripeptide aminopeptidase LTA4H, 3.4.11.4, LTA4H, LTA4

Purification

Purified by antigen affinity column.

Accession

P09960

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Leukotriene A-4 hydrolase (LTA4H) is a ~69 kDa protein. Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4). Can utilize LTA5 less effectively as a substrate than LTA4, and produce LTB5. Also has aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL).

1. Rudberg, PC. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 4215-20. PMID: 11917124

2. Thunnissen, MM. et al. (2002) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 16, 1648-50. PMID: 12207002

3. Rudberg, PC. et al. (2004) The Journal of biological chemistry 279, 27376-82. PMID: 15078870

4. Tholander, F. et al. (2008) Chemistry & biology 15, 920-9. PMID: 18804029

5. Odlander, B. et al. (1991) Archives of biochemistry and biophysics 287, 167-74. PMID: 1897988

6. Toh, H. et al. (1990) Biochemical and biophysical research communications 171, 216-21. PMID: 1975494

7. Haeggström, JZ. et al. (1990) Biochemical and biophysical research communications 172, 965-70. PMID: 2244921

8. Nathaniel, DJ. et al. (1985) Biochemical and biophysical research communications 131, 827-35. PMID: 2996528

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-LTA4H polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate

References

Datasheet

Anti-Human LTA4H Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human LTA4H Polyclonal Antibody (HY412014)

Overview

Images

References

Datasheet

SDS

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