Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

Name: Anti-Human MAD2L2 Polyclonal Antibody
Brand: abinScience
SKU: HP994014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP994014

Description

Anti-Human MAD2L2 Polyclonal Antibody (HP994014) is a rabbit polyclonal antibody detecting MAD2L2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAD2L2 (Met1-Ser211).

Target

Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B, MAD2L2, MAD2-like protein 2, hREV7, REV7, REV7 homolog, MAD2B

Purification

Purified by antigen affinity column.

Accession

Q9UI95

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitotic spindle assembly checkpoint protein MAD2B (MAD2L2) is a ~24 kDa protein. Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection.

1. Pfleger, CM. et al. (2001) Genes & development 15, 1759-64. PMID: 11459825

2. Chen, J. et al. (2001) Genes & development 15, 1765-70. PMID: 11459826

3. Zhang, L. et al. (2007) Molecular and cellular biology 27, 2861-9. PMID: 17296730

4. Iwai, H. et al. (2007) Cell 130, 611-23. PMID: 17719540

5. Hong, CF. et al. (2009) The Journal of biological chemistry 284, 19613-22. PMID: 19443654

6. Gupta, R. et al. (2018) Cell 173, 972-988.e23. PMID: 29656893

7. Hara, K. et al. (2010) The Journal of biological chemistry 285, 12299-307. PMID: 20164194

8. Xie, W. et al. (2012) Protein & cell 3, 864-74. PMID: 23143872

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MAD2L2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate

References

Datasheet

Anti-Human MAD2L2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human MAD2L2 Polyclonal Antibody (HP994014)

Overview

Images

References

Datasheet

SDS

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