Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Name: Anti-Human MAGEA3 Recombinant Antibody (SAA1562)
Brand: abinScience
SKU: HW798013
Price: 110 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW798013

Species reactivity

Human

Applications

ELISA, FCM

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1562

Clonality

Monoclonal

Target

Antigen MZ2-D, MAGE3, Melanoma-associated antigen 3, CT1.3, MAGE-3 antigen, Cancer/testis antigen 1.3, MAGEA3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P43357

RRID

Anti-Human MAGEA3 Antibody (SAA1562) (abinScience Cat# HW798013, RRID:AB_3727323)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Melanoma-associated antigen 3 (MAGEA3) is a ~34 kDa protein. Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines.

1. Doyle, JM. et al. (2010) Molecular cell 39, 963-74. PMID: 20864041

2. Ravichandran, R. et al. (2019) EMBO reports 20, e47352. PMID: 31267705

3. Yang, B. et al. (2007) Cancer research 67, 9954-62. PMID: 17942928

4. Gaugler, B. et al. (1994) The Journal of experimental medicine 179, 921-30. PMID: 8113684

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MAGEA3 Antibody (SAA1562)
Flow cytometry

Flow-cytometry using anti-human MAGEA3 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MAGEA3 monoclonal antibody (Catalog HW798013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human MAGEA3 Recombinant Antibody (SAA1562).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MAGEA3 Recombinant Antibody (SAA1562) (HW798013)

Overview

Images

References

Datasheet

SDS

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