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Anti-Human Malin Polyclonal Antibody (HA130014)

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Anti-Human Malin Polyclonal Antibody
Anti-Human Malin Polyclonal Antibody
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Overview
Catalog No.HA130014
Description
Anti-Human Malin Polyclonal Antibody (HA130014) is a rabbit polyclonal antibody detecting Malin. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human Malin (Gly112-Gly395).
Target E3 ubiquitin-protein ligase NHLRC1, EC:2.3.2.27, EPM2B, Malin, NHL repeat-containing protein 1, NHLRC1, RING-type E3 ubiquitin transferase NHLRC1
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6VVB1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

E3 ubiquitin-protein ligase NHLRC1 (Malin) is a ~42 kDa protein. E3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation.

Note For research use only
Images
  • Anti-Human Malin Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NHLRC1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse heart lysate

References
Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human Malin Polyclonal Antibody

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