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Anti-Human MAP1B Polyclonal Antibody (HW426014)

Anti-Human MAP1B Polyclonal Antibody
Anti-Human MAP1B Polyclonal Antibody
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Overview
Catalog No.HW426014
Description
Anti-Human MAP1B Polyclonal Antibody (HW426014) is a rabbit polyclonal antibody detecting MAP1B in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, and Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MAP1B (Lys414-Glu567).
Target MAP1B
Purification Purified by antigen affinity column.
Accession P46821
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesMicrotubule-associated protein 1B, MAP-1B, MAP1B heavy chain, MAP1 light chain LC1, MAP1B
Background

Microtubule-associated protein 1B (MAP1B) is a ~270 kDa protein. Facilitates tyrosination of alpha-tubulin in neuronal microtubules. Phosphorylated MAP1B is required for proper microtubule dynamics and plays a role in the cytoskeletal changes that accompany neuronal differentiation and neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.

1. Cui, L. et al. (2020) JCI insight 5. PMID: 33268592
Note For research use only.
Images
  • Anti-Human MAP1B Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MAP1B polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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