Anti-Human MAPK4 Polyclonal Antibody (HB842014)

Name: Anti-Human MAPK4 Polyclonal Antibody
Brand: abinScience
SKU: HB842014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB842014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MAPK4 (Met1-Ser313).

Target

MAP kinase 4, Extracellular signal-regulated kinase 4, ERK4, MAPK 4, Mitogen-activated protein kinase 4, MAPK4, ERK-4, PRKM4, MAP kinase isoform p63, p63-MAPK

Purification

Purified by antigen affinity column.

Accession

P31152

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitogen-activated protein kinase 4 (MAPK4) is a ~65 kDa protein. Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human MAPK4 polyclonal antibody at 1ug/mL on recombinant Human MAPK4 (Catalog No. HB842012).

References

Datasheet

Anti-Human MAPK4 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MAPK4 Polyclonal Antibody (HB842014)

Overview

Images

References

Datasheet

SDS

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