Anti-Human MARCKS Polyclonal Antibody (HB645014)

Name: Anti-Human MARCKS Polyclonal Antibody
Brand: abinScience
SKU: HB645014
Price: 168 USD
Availability: InStock

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100ug

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB645014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MARCKS (Lys155-Gly199).

Target

Myristoylated alanine-rich C-kinase substrate, 80K-L protein, PKCSL, Protein kinase C substrate, 80 kDa protein, light chain, PRKCSL, MACS, MARCKS

Purification

Purified by antigen affinity column.

Accession

P29966

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Myristoylated alanine-rich C-kinase substrate (MARCKS) is a ~31 kDa protein. Membrane-associated protein that plays a role in the structural modulation of the actin cytoskeleton, chemotaxis, motility, cell adhesion, phagocytosis, and exocytosis through lipid sequestering and/or protein docking to membranes. Thus, exerts an influence on a plethora of physiological processes, such as embryonic development, tissue regeneration, neuronal plasticity, and inflammation. Sequesters phosphatidylinositol 4,5-bisphosphate (PIP2) at lipid rafts in the plasma membrane of quiescent cells, an action reversed by protein kinase C, ultimately inhibiting exocytosis. During inflammation, promotes the migration and adhesion of inflammatory cells and the secretion of cytokines such as tumor necrosis factor (TNF), particularly in macrophages. Plays an essential role in bacteria-induced intracellular reactive oxygen species (ROS) formation in the monocytic cell type.

1. Rodriguez Peña, MJ. et al. (2013) PloS one 8, e64551. PMID: 23704996

2. Huber, R. et al. (2022) Antioxidants (Basel, Switzerland) 11. PMID: 36009319

3. Issara-Amphorn, J. et al. (2023) Scientific reports 13, 19562. PMID: 37949888

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human MARCKS polyclonal antibody at 1ug/mL on recombinant Human MARCKS (Catalog No. HB645022).

References

Datasheet