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Anti-Human MARK4 Polyclonal Antibody (HA382014)

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Overview
Catalog No.HA382014
Description
Anti-Human MARK4 Polyclonal Antibody (HA382014) is a rabbit polyclonal antibody detecting MARK4 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MARK4 (Gly57-Lys370).
Target MAP/microtubule affinity-regulating kinase 4, 2.7.11.1, MAP/microtubule affinity-regulating kinase-like 1, MARK4, KIAA1860, MARKL1
Purification Purified by antigen affinity column.
Accession Q96L34
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

MAP/microtubule affinity-regulating kinase 4 (MARK4) is a ~82 kDa protein. Serine/threonine-protein kinase. Phosphorylates the microtubule-associated protein MAPT/TAU. Also phosphorylates the microtubule-associated proteins MAP2 and MAP4. Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles. Required for the initiation of axoneme extension during cilium assembly.

1. Trinczek, B. et al. (2004) The Journal of biological chemistry 279, 5915-23. PMID: 14594945
2. Schneider, A. et al. (2004) Journal of neurochemistry 88, 1114-26. PMID: 15009667
3. Li, L. et al. (2013) The Journal of biological chemistry 288, 703-8. PMID: 23184942
4. Gu, GJ. et al. (2013) Neuromolecular medicine 15, 458-69. PMID: 23666762
5. Rovina, D. et al. (2014) European journal of cell biology 93, 355-65. PMID: 25123532
6. Kuhns, S. et al. (2013) The Journal of cell biology 200, 505-22. PMID: 23400999
7. Li, X. et al. (2017) Nature communications 8, 15986. PMID: 28656979
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human MARK4 Polyclonal Antibody

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