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Anti-Human MAT2B Polyclonal Antibody (HP063014)

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Overview
Catalog No.HP063014
Description
Anti-Human MAT2B Polyclonal Antibody (HP063014) is a rabbit polyclonal antibody detecting MAT2B in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MAT2B (Glu21-His334).
Target TGR, Methionine adenosyltransferase 2 subunit beta, MAT II beta, MAT2B, Methionine adenosyltransferase II beta, Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase
Purification Purified by antigen affinity column.
Accession Q9NZL9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Methionine adenosyltransferase 2 subunit beta (MAT2B) is a ~37 kDa protein. Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine. Can bind NADP (in vitro).

1. LeGros, HL. et al. (2000) The Journal of biological chemistry 275, 2359-66. PMID: 10644686
2. González, B. et al. (2012) PloS one 7, e50329. PMID: 23189196
3. Murray, B. et al. (2014) IUCrJ 1, 240-9. PMID: 25075345
4. Shafqat, N. et al. (2013) The Biochemical journal 452, 27-36. PMID: 23425511
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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