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Anti-Human MBIP Polyclonal Antibody (HA383014)

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Overview
Catalog No.HA383014
Description
Anti-Human MBIP Polyclonal Antibody (HA383014) is a rabbit polyclonal antibody detecting MBIP in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MBIP (Leu283-Glu334).
Target MAP3K12-binding inhibitory protein 1, MAPK upstream kinase-binding inhibitory protein, MBIP, MUK-binding inhibitory protein
Purification Purified by antigen affinity column.
Accession Q9NS73
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

MAP3K12-binding inhibitory protein 1 (MBIP) is a ~39 kDa protein. Functions as part of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Inhibits the MAP3K12 activity to induce the activation of the JNK/SAPK pathway. Together with MOCS2B, inhibits the activity of stress kinase EIF2AK2/PKR; this may lead to effects such as suppression of JNK activation and subsequent stress-responsive transcription, or suppression of eIF2a phosphorylation to favor translation.

1. Guelman, S. et al. (2009) Molecular and cellular biology 29, 1176-88. PMID: 19103755
2. Fukuyama, K. et al. (2000) The Journal of biological chemistry 275, 21247-54. PMID: 10801814
3. Suganuma, T. et al. (2016) Journal of molecular cell biology 8, 44-50. PMID: 26705305
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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