Anti-Human MBTD1 IP Recombinant Antibody (SAA0210) (HC542013)

Name: Anti-Human MBTD1 IP Recombinant Antibody (SAA0210)
Brand: abinScience
SKU: HC542013
Price: 191 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC542013

Species reactivity

Human

Applications

ELISA, IF, IP, IP-MS

Host species

Mouse

Isotype

IgG

Clone ID

SAA0210

Clonality

Monoclonal

Target

MBTD1, MBT domain-containing protein 1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q05BQ5

RRID

Anti-Human MBTD1 IP Antibody (SAA0210) (abinScience Cat# HC542013, RRID:AB_3729744)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

MBT domain-containing protein 1 (MBTD1) is a ~70 kDa protein. Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR). MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively). In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me. Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR).

1. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

2. Zhang, H. et al. (2020) Cell reports 30, 3996-4002.e4. PMID: 32209463

3. Eryilmaz, J. et al. (2009) PloS one 4, e7274. PMID: 19841675

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human MBTD1 IP Antibody (SAA0210)

References

Datasheet

Anti-Human MBTD1 IP Recombinant Antibody (SAA0210).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MBTD1 IP Recombinant Antibody (SAA0210) (HC542013)

Overview

Images

References

Datasheet

SDS

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