Anti-Human MCM6 Polyclonal Antibody (HC528014)

Name: Anti-Human MCM6 Polyclonal Antibody
Brand: abinScience
SKU: HC528014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC528014

Description

Anti-Human MCM6 Polyclonal Antibody (HC528014) is a rabbit polyclonal antibody detecting MCM6 in ELISA, IHC, WB. Suitable for Rat, Bovine, Human, Mouse, and Xenopus laevis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MCM6 (Arg190-Thr661).

Target

DNA replication licensing factor MCM6, p105MCM, MCM6

Purification

Purified by antigen affinity column.

Accession

Q14566

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA replication licensing factor MCM6 is a ~92 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

2. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

3. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

4. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

5. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

6. Ishimi, Y. (1997) The Journal of biological chemistry 272, 24508-13. PMID: 9305914

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MCM6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HL60 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: PC12 cell lysate
Lane 6: NIH3T3 cell lysate
Lane 7: Mouse spleen lysate