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Anti-Human MED30 Polyclonal Antibody (HP922014)

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Overview
Catalog No.HP922014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MED30 (Asn29-Gly105).
Target MED30, THRAP6, Mediator complex subunit 30, Thyroid hormone receptor-associated protein 6, TRAP25, TRAP/Mediator complex component TRAP25, Trap25, Mediator of RNA polymerase II transcription subunit 30, Thyroid hormone receptor-associated protein complex 25 kDa component
Purification Purified by antigen affinity column.
Accession Q96HR3
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mediator of RNA polymerase II transcription subunit 30 (MED30) is a ~20 kDa protein. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Note For research use only.
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Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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