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Anti-Human METTL5 Polyclonal Antibody (HA273014)

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Anti-Human METTL5 Polyclonal Antibody
Anti-Human METTL5 Polyclonal Antibody
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Overview
Catalog No.HA273014
Description
Anti-Human METTL5 Polyclonal Antibody (HA273014) is a rabbit polyclonal antibody detecting METTL5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human METTL5 (Met1-Phe209).
Target Methyltransferase-like protein 5, METTL5, rRNA N6-adenosine-methyltransferase METTL5
Purification Purified by antigen affinity column.
Accession Q9NRN9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

rRNA N(6)-adenosine-methyltransferase METTL5 is a ~23 kDa protein. Catalytic subunit of a heterodimer with TRMT112, which specifically methylates the 6th position of adenine in position 1832 of 18S rRNA. N6-methylation of adenine(1832) in 18S rRNA resides in the decoding center of 18S rRNA and is required for translation and embryonic stem cells (ESCs) pluripotency and differentiation.

1. van Tran, N. et al. (2019) Nucleic acids research 47, 7719-7733. PMID: 31328227
2. Ignatova, VV. et al. (2020) Genes & development 34, 715-729. PMID: 32217665
3. Rong, B. et al. (2020) Cell reports 33, 108544. PMID: 33357433
4. Yu, D. et al. (2021) The Journal of biological chemistry 296, 100270. PMID: 33428944
5. Sepich-Poore, C. et al. (2022) The Journal of biological chemistry 298, 101590. PMID: 35033535
Note For research use only.
Images
  • Anti-Human METTL5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-METTL5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human METTL5 Polyclonal Antibody

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