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Anti-Human MIEF1 Polyclonal Antibody (HP379014)

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Overview
Catalog No.HP379014
Description
Anti-Human MIEF1 Polyclonal Antibody (HP379014) is a rabbit polyclonal antibody detecting MIEF1 in ELISA, IHC, WB. Suitable for Human, Mouse, Pongo abelii, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MIEF1 (Ala119-Thr463).
Target MID51, SMCR7-like protein, Mitochondrial dynamics protein MID51, MIEF1, Mitochondrial elongation factor 1, Smith-Magenis syndrome chromosomal region candidate gene 7 protein-like, Mitochondrial dynamics protein of 51 kDa, SMCR7L
Purification Purified by antigen affinity column.
Accession Q9NQG6
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mitochondrial dynamics protein MIEF1 is a ~51 kDa protein. Mitochondrial outer membrane protein which regulates mitochondrial fission/fusion dynamics. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP.

1. Zhao, J. et al. (2011) The EMBO journal 30, 2762-78. PMID: 21701560
2. Palmer, CS. et al. (2013) The Journal of biological chemistry 288, 27584-27593. PMID: 23921378
3. Charif, M. et al. (2021) Molecular neurodegeneration 16, 12. PMID: 33632269
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human MIEF1 Polyclonal Antibody

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