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Anti-Human MIEF2 Polyclonal Antibody (HP241014)

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Overview
Catalog No.HP241014
Description
Anti-Human MIEF2 Polyclonal Antibody (HP241014) is a rabbit polyclonal antibody detecting MIEF2 in ELISA, IHC, WB. Suitable for Human and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MIEF2 (Thr126-Leu454).
Target MID49, Smith-Magenis syndrome chromosomal region candidate gene 7 protein, MIEF2, Mitochondrial dynamics protein of 49 kDa, SMCR7, Mitochondrial dynamics protein MID49, Mitochondrial elongation factor 2
Purification Purified by antigen affinity column.
Accession Q96C03
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Mitochondrial dynamics protein MID49 (MIEF2) is a ~49 kDa protein. Mitochondrial outer membrane protein involved in the regulation of mitochondrial organization. It is required for mitochondrial fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.

1. Bartsakoulia, M. et al. (2018) Human molecular genetics 27, 1186-1195. PMID: 29361167
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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