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Anti-Human MMAB Polyclonal Antibody (HA993014)

Anti-Human MMAB Polyclonal Antibody
Anti-Human MMAB Polyclonal Antibody
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Overview
Catalog No.HA993014
Description
Anti-Human MMAB Polyclonal Antibody (HA993014) is a rabbit polyclonal antibody detecting MMAB in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MMAB (Met1-Ala186).
Target MMAB, Methylmalonic aciduria type B protein, Cob(II)yrinic acid a, c-diamide adenosyltransferase, Cobinamide/cobalamin adenosyltransferase, Corrinoid adenosyltransferase, Cob(II)alamin adenosyltransferase
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q96EY8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Corrinoid adenosyltransferase MMAB is a ~27 kDa protein. Converts cob(I)alamin to adenosylcobalamin (adenosylcob(III)alamin), a coenzyme for methylmalonyl-CoA mutase, therefore participates in the final step of the vitamin B12 conversion. Generates adenosylcobalamin (AdoCbl) and directly delivers the cofactor to MUT in a transfer that is stimulated by ATP-binding to MMAB and gated by MMAA (Probable).

1. Leal, NA. et al. (2003) The Journal of biological chemistry 278, 9227-34. PMID: 12514191
Note For research use only.
Images
  • Anti-Human MMAB Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-RBBP9 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: BxPC-3 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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