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Anti-Human MMUT Polyclonal Antibody (HB246014)

Anti-Human MMUT Polyclonal Antibody
Anti-Human MMUT Polyclonal Antibody
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Overview
Catalog No.HB246014
Description
Anti-Human MMUT Polyclonal Antibody (HB246014) is a rabbit polyclonal antibody detecting MMUT in ELISA, IHC, WB. Suitable for Macaca fascicularis, Mus musculus, Human, Sus scrofa, and Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human MMUT (Val510-Ser749).
Target MMUT
Purification Purified by antigen affinity column.
Accession P22033
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesMethylmalonyl-CoA mutase, mitochondrial, MCM, 5.4.99.2, Methylmalonyl-CoA isomerase, MMUT, MUT
Background

Methylmalonyl-CoA mutase, mitochondrial (MMUT) is a ~83 kDa protein. Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.

Note For research use only.
Images
  • Anti-Human MMUT Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MMUT polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Mouse kidney lysate
    Lane 3: Mouse liver lysate
    Lane 4: Rat heart lysate
    Lane 5: Rat kidney lysate
    Lane 6: Rat liver lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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