Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014)

Name: Anti-Human MPHOSPH10 Polyclonal Antibody
Brand: abinScience
SKU: HT215014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HT215014

Description

Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014) is a rabbit polyclonal antibody detecting MPHOSPH10 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human MPHOSPH10 (Asp461-Leu681).

Target

U3 small nucleolar ribonucleoprotein protein MPP10, M phase phosphoprotein 10, MPHOSPH10, MPP10

Purification

Purified by antigen affinity column.

Accession

O00566

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) is a ~78 kDa protein. Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.

1. Granneman, S. et al. (2003) Nucleic acids research 31, 1877-87. PMID: 12655004

2. Singh, S. et al. (2021) Science (New York, N.Y.) 373, eabj5338. PMID: 34516797

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MPHOSPH10 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate

References

Datasheet

Anti-Human MPHOSPH10 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MPHOSPH10 Polyclonal Antibody (HT215014)

Overview

Images

References

Datasheet

SDS

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