Anti-Human MRPL45 Polyclonal Antibody (HP020014)

Name: Anti-Human MRPL45 Polyclonal Antibody
Brand: abinScience
SKU: HP020014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP020014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MRPL45 (Glu92-Ile265).

Target

Mitochondrial large ribosomal subunit protein mL45, MRPL45, 39S ribosomal protein L45, mitochondrial, L45mt, MRP-L45

Purification

Purified by antigen affinity column.

Accession

Q9BRJ2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Large ribosomal subunit protein mL45 (MRPL45) is a ~35 kDa protein. Component of the mitochondrial large ribosomal subunit (mt-LSU). Within the mitochondrial ribosomes, required to direct the nascent polypeptide toward the tunnel exit and position the exit at a distance from the membrane surface.

1. Brown, A. et al. (2014) Science (New York, N.Y.) 346, 718-722. PMID: 25278503

2. Amunts, A. et al. (2015) Science (New York, N.Y.) 348, 95-98. PMID: 25838379

3. Brown, A. et al. (2017) Nature structural & molecular biology 24, 866-869. PMID: 28892042

4. Itoh, Y. et al. (2021) Science (New York, N.Y.) 371, 846-849. PMID: 33602856

5. Rebelo-Guiomar, P. et al. (2022) Nature communications 13, 929. PMID: 35177605

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MRPL45 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse heart lysate
Lane 2: Rat brain lysate
Lane 3: Rat heart lysate

References

Datasheet

Anti-Human MRPL45 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MRPL45 Polyclonal Antibody (HP020014)

Overview

Images

References

Datasheet

SDS

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