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Anti-Human MRPS27 Polyclonal Antibody (HP970014)

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Overview
Catalog No.HP970014
Description
Anti-Human MRPS27 Polyclonal Antibody (HP970014) is a rabbit polyclonal antibody detecting MRPS27 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human MRPS27 (Ser32-Ala414).
Target Mitochondrial ribosomal protein S27, MRP-S27, 28S ribosomal protein S27, mitochondrial, KIAA0264, MRPS27, Mitochondrial small ribosomal subunit protein mS27, S27mt
Purification Purified by antigen affinity column.
Accession Q92552
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Small ribosomal subunit protein mS27 (MRPS27) is a ~47 kDa protein. RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Involved also in positive regulation of cell proliferation and tumor cell growth.

1. Davies, SM. et al. (2012) FEBS letters 586, 3555-61. PMID: 22841715
2. Pu, M. et al. (2017) Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 39, 1010428317709127. PMID: 28714366
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human MRPS27 Polyclonal Antibody

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