



| Catalog No. | HC496107 |
|---|---|
| Species reactivity | Human |
| Applications | FCM, IHC |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a, lambda |
| Clone ID | SAA0145 |
| Tested applications | FCM |
| Target | CAK1 antigen, MPF, MSLN, Megakaryocyte-potentiating factor, Mesothelin, Mesothelin, cleaved form, Pre-pro-megakaryocyte-potentiating factor |
| Endotoxin level | < 10 EU/mg |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Accession | Q13421 |
| Form | Liquid |
| Storage buffer | 0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Background | Mesothelin (MSLN) is a ~68 kDa protein. Membrane-anchored forms may play a role in cellular adhesion. |
| Note | For research use only. |

Flow-cytometry using anti-human MSLN antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MSLN antibody monoclonal antibody (Catalog # HC496107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human MSLN antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human MSLN monoclonal antibody (Catalog: HC496107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.




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