Anti-Human MTAP Polyclonal Antibody (HC278014)

Name: Anti-Human MTAP Polyclonal Antibody
Brand: abinScience
SKU: HC278014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC278014

Description

Anti-Human MTAP Polyclonal Antibody (HC278014) is a rabbit polyclonal antibody detecting MTAP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Macaca mulatta.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human MTAP (Met1-His283).

Target

MTAP, S-methyl-5'-thioadenosine phosphorylase, MSAP, MTA phosphorylase, 5'-methylthioadenosine phosphorylase, MTAPase

Purification

Purified by antigen affinity column.

Accession

Q13126

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

S-methyl-5'-thioadenosine phosphorylase (MTAP) is a ~31 kDa protein. Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MTAP polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: Raji cell lysate
Lane 4: Hela cell lysate
Lane 5: HCT116 cell lysate

References

Datasheet

Anti-Human MTAP Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MTAP Polyclonal Antibody (HC278014)

Overview

Images

References

Datasheet

SDS

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