Anti-Human MTHFD1 Polyclonal Antibody (HY454014)

Name: Anti-Human MTHFD1 Polyclonal Antibody
Brand: abinScience
SKU: HY454014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY454014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human MTHFD1 (Met1-Lys297).

Target

MTHFC, C1-THF synthase, MTHFD1, C-1-tetrahydrofolate synthase, cytoplasmic, MTHFD

Purification

Purified by antigen affinity column.

Accession

P11586

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

C-1-tetrahydrofolate synthase, cytoplasmic (MTHFD1) is a ~101 kDa protein. Trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. These derivatives of tetrahydrofolate are differentially required in nucleotide and amino acid biosynthesis, (6S)-10-formyltetrahydrofolate being required for purine biosynthesis while (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate is used for serine and methionine biosynthesis for instance.

1. Schmidt, A. et al. (2000) Biochemistry 39, 6325-35. PMID: 10828945

2. Christensen, KE. et al. (2009) Human mutation 30, 212-20. PMID: 18767138

3. Hum, DW. et al. (1991) Protein engineering 4, 493-500. PMID: 1881876

4. Burda, P. et al. (2015) Journal of inherited metabolic disease 38, 863-72. PMID: 25633902

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-MTHFD1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Hela cell lysate
Lane 5: A549 cell lysate
Lane 6: Mouse kidney lysate
Lane 7: Mouse liver lysate
Lane 8: Rat liver lysate

References

Datasheet

Anti-Human MTHFD1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human MTHFD1 Polyclonal Antibody (HY454014)

Overview

Images

References

Datasheet

SDS

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